Oral Intake of Low-Molecular-Weight Collagen Peptide Improves Hydration, Elasticity, and Wrinkling in Human Skin: A Randomized, Double-Blind, Placebo-Controlled Study

Nutrients. 2018 Jun 26;10(7):826. doi: 10.3390/nu10070826.


Collagen-peptide supplementation could be an effective remedy to improve hydration, elasticity, and wrinkling in human skin. The aim of this study was to conduct a double-blind, randomized, placebo-controlled trial to clinically evaluate the effect on human skin hydration, wrinkling, and elasticity of Low-molecular-weight Collagen peptide (LMWCP) with a tripetide (Gly-X-Y) content >15% including 3% Gly-Pro-Hyp. Individuals (n = 64) were randomly assigned to receive either placebo or 1000 mg of LMWCP once daily for 12 weeks. Parameters of skin hydration, wrinkling, and elasticity were assessed at baseline and after 6 weeks and 12 weeks. Compared with the placebo group, skin-hydration values were significantly higher in the LMWCP group after 6 weeks and 12 weeks. After 12 weeks in the LMWCP group, visual assessment score and three parameters of skin wrinkling were significantly improved compared with the placebo group. In case of skin elasticity, one parameter out of three was significantly improved in the LMWCP group from the baseline after 12 weeks, while, compared with the placebo group, two parameters out of three in the LMWCP group were higher with significance after 12 weeks. In terms of the safety of LMWCP, none of the subjects presented adverse symptoms related to the test material during the study period. These results suggest that LMWCP can be used as a health functional food ingredient to improve human skin hydration, elasticity, and wrinkling.

Keywords: collagen hydrolysate; collagen tripeptide; fish collagen; low-molecular-weight collagen peptide; photoaging; skin elasticity; skin hydration; skin wrinkling; type I collagen.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Administration, Oral
  • Adult
  • Collagen Type I / administration & dosage*
  • Collagen Type I / adverse effects
  • Dietary Supplements* / adverse effects
  • Double-Blind Method
  • Elasticity
  • Female
  • Humans
  • Middle Aged
  • Molecular Weight
  • Oligopeptides / administration & dosage*
  • Oligopeptides / adverse effects
  • Peptide Fragments / administration & dosage*
  • Peptide Fragments / adverse effects
  • Protein Hydrolysates / administration & dosage*
  • Protein Hydrolysates / adverse effects
  • Skin / drug effects*
  • Skin / metabolism
  • Skin Aging / drug effects*
  • Time Factors
  • Treatment Outcome
  • Water / metabolism


  • Collagen Type I
  • Oligopeptides
  • Peptide Fragments
  • Protein Hydrolysates
  • glycyl-prolyl-hydroxyproline
  • Water