Cryo-EM structure of an essential Plasmodium vivax invasion complex

Nature. 2018 Jul;559(7712):135-139. doi: 10.1038/s41586-018-0249-1. Epub 2018 Jun 27.


Plasmodium vivax is the most widely distributed malaria parasite that infects humans1. P. vivax invades reticulocytes exclusively, and successful entry depends on specific interactions between the P. vivax reticulocyte-binding protein 2b (PvRBP2b) and transferrin receptor 1 (TfR1)2. TfR1-deficient erythroid cells are refractory to invasion by P. vivax, and anti-PvRBP2b monoclonal antibodies inhibit reticulocyte binding and block P. vivax invasion in field isolates2. Here we report a high-resolution cryo-electron microscopy structure of a ternary complex of PvRBP2b bound to human TfR1 and transferrin, at 3.7 Å resolution. Mutational analyses show that PvRBP2b residues involved in complex formation are conserved; this suggests that antigens could be designed that act across P. vivax strains. Functional analyses of TfR1 highlight how P. vivax hijacks TfR1, an essential housekeeping protein, by binding to sites that govern host specificity, without affecting its cellular function of transporting iron. Crystal and solution structures of PvRBP2b in complex with antibody fragments characterize the inhibitory epitopes. Our results establish a structural framework for understanding how P. vivax reticulocyte-binding protein engages its receptor and the molecular mechanism of inhibitory monoclonal antibodies, providing important information for the design of novel vaccine candidates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / pharmacology
  • Antigens, CD / chemistry
  • Antigens, CD / genetics
  • Antigens, CD / metabolism
  • Antigens, CD / ultrastructure
  • Binding Sites
  • Cryoelectron Microscopy*
  • Humans
  • Malaria Vaccines / immunology
  • Models, Molecular
  • Mutation
  • Plasmodium vivax / chemistry*
  • Plasmodium vivax / cytology
  • Plasmodium vivax / genetics
  • Plasmodium vivax / ultrastructure*
  • Protozoan Proteins / antagonists & inhibitors
  • Protozoan Proteins / chemistry*
  • Protozoan Proteins / genetics
  • Protozoan Proteins / ultrastructure*
  • Receptors, Transferrin / chemistry
  • Receptors, Transferrin / genetics
  • Receptors, Transferrin / metabolism
  • Receptors, Transferrin / ultrastructure
  • Reticulocytes / metabolism
  • Structure-Activity Relationship
  • Transferrin / chemistry
  • Transferrin / metabolism
  • Transferrin / ultrastructure


  • Antibodies, Monoclonal
  • Antigens, CD
  • CD71 antigen
  • Malaria Vaccines
  • Protozoan Proteins
  • Receptors, Transferrin
  • Transferrin