Toll-like receptors 4, 5, 6 and 7 are constitutively expressed in non-human primate retinal neurons

J Neuroimmunol. 2018 Sep 15:322:26-35. doi: 10.1016/j.jneuroim.2018.06.007. Epub 2018 Jun 11.

Abstract

The purpose of this study was to characterize cell-specific expression patterns of Toll-like receptors (TLR) in non-human primate (NHP) neural retina tissue. TLR 4, 5, 6, and 7 proteins were detected by immunblotting of macaque retina tissue lysates and quantitative PCR (qPCR) demonstrated TLRs 4-7 mRNA expression. Immunofluorescence (IF) microscopy detected TLRs 4-7 in multiple cell types in macaque neural retina including Muller, retinal ganglion cells (RGC), amacrine, and bipolar cells. These results demonstrate that TLRs 4-7 are constitutively expressed by neurons in the NHP retina raising the possibility that these cells could be involved in retinal innate inflammatory responses.

Keywords: Inflammation; Innate immunity; Non-human primates; Retina; Toll-like receptors.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amacrine Cells / metabolism
  • Animals
  • Blotting, Western
  • Ependymoglial Cells / metabolism
  • Eye Proteins / biosynthesis*
  • Eye Proteins / genetics
  • Gene Expression Regulation, Developmental
  • Immunity, Innate
  • Macaca fascicularis / metabolism*
  • Macaca mulatta / metabolism*
  • Neurons / metabolism*
  • Polymerase Chain Reaction
  • Retina / cytology*
  • Retina / immunology
  • Retina / metabolism
  • Retinal Bipolar Cells / metabolism
  • Retinal Ganglion Cells / metabolism
  • Toll-Like Receptors / biosynthesis*
  • Toll-Like Receptors / genetics

Substances

  • Eye Proteins
  • Toll-Like Receptors