A Fungal Protease Model to Interrogate Allergic Lung Immunity

Methods Mol Biol. 2018;1799:1-9. doi: 10.1007/978-1-4939-7896-0_1.


Allergic airway diseases (asthma and chronic rhinosinusitis) are among the most common of all human diseases in heavily industrialized societies. Animal models of asthma have provided remarkable insight into allergic disease pathogenesis and will continue to drive the discovery of new therapeutic insights. We provide in this chapter a detailed protocol for inducing allergic immunity in the lungs of mice using a purified fungal protease and include related protocols for assessing immune endpoints.

Keywords: Airway hyperresponsiveness; Allergic immunity; Asthma; Fungi; Protease; TH2 cell.

MeSH terms

  • Allergens / immunology*
  • Animals
  • Biomarkers
  • Bronchoalveolar Lavage Fluid
  • Disease Models, Animal
  • Fungi / enzymology
  • Fungi / immunology*
  • Hypersensitivity / etiology*
  • Hypersensitivity / pathology
  • Immunity*
  • Lung / immunology*
  • Lung / pathology
  • Mice
  • Peptide Hydrolases / immunology*
  • Plethysmography
  • Th2 Cells / immunology
  • Th2 Cells / metabolism


  • Allergens
  • Biomarkers
  • Peptide Hydrolases