The Generation and Use of Allergen-Specific TCR Transgenic Animals

Methods Mol Biol. 2018:1799:183-210. doi: 10.1007/978-1-4939-7896-0_15.

Abstract

The generation of allergen-specific TCR transgenic animals allows for the characterization of allergen-specific T-cell responses in vivo and in vitro and is a powerful tool to study adaptive immunity to allergens. Here we describe an approach starting from the isolation of antigen-specific T-cell hybridomas and using PCR, flow cytometric, and co-culture methods to obtain antigen-specific MHC class II-restricted CD4+ TCR transgenic mice on the Rag2-/- background.

Keywords: Allergen; Hybridoma; T-cell fusion; T-cell receptor; Transgenic.

MeSH terms

  • Allergens / immunology*
  • Animals
  • CD4-Positive T-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / metabolism
  • Cell Communication / immunology
  • Cell Line
  • Cloning, Molecular
  • Dendritic Cells / immunology
  • Dendritic Cells / metabolism
  • Gene Expression
  • Gene Order
  • Genetic Vectors / genetics
  • Lymph Nodes / innervation
  • Lymph Nodes / metabolism
  • Mice
  • Mice, Transgenic
  • Receptors, Antigen, T-Cell / genetics
  • Receptors, Antigen, T-Cell / metabolism*
  • T-Cell Antigen Receptor Specificity / genetics
  • T-Cell Antigen Receptor Specificity / immunology*
  • T-Lymphocyte Subsets / immunology*
  • T-Lymphocyte Subsets / metabolism*

Substances

  • Allergens
  • Receptors, Antigen, T-Cell