Pulmonary dendritic cells (DCs) are potent antigen-presenting cells that can activate both naïve and memory/effector T cells. However, very little is known of how movements and localization of DCs contribute to these events. To study this, we have developed new procedures that combine precision-cut lung slices with cell staining using fluorescently tagged antibodies to detect individual cell types. In this chapter, we describe these methods in detail and show how they can be used to study the localization of not only DCs but also other leukocytes of interest, as well as structural cells within the lung.
Keywords: Airway epithelium; Antigen-presenting cells; Confocal microscopy; Dendritic cells; Epithelial cells; Live-cell imaging; Localization; Lung; Lymphatics; Macrophages; Precision-cut lung slices.