Production of a highly concentrated probiotic culture of Lactococcus lactis CECT 539 containing high amounts of nisin

Mónica Costas Malvido; Elisa Alonso González; David Outeiriño; Nelson Pérez Guerra

doi:10.1007/s13205-018-1318-8

Production of a highly concentrated probiotic culture of Lactococcus lactis CECT 539 containing high amounts of nisin

3 Biotech. 2018 Jul;8(7):292. doi: 10.1007/s13205-018-1318-8. Epub 2018 Jun 25.

Authors

Mónica Costas Malvido¹, Elisa Alonso González¹, David Outeiriño², Nelson Pérez Guerra¹

Affiliations

¹ 1Department of Analytical and Food Chemistry, Food Science and Technology Faculty, University of Vigo, Ourense Campus, As Lagoas s/n, 32004 Ourense, Spain.
² 2Department of Chemical Engineering, Faculty of Sciences, University of Vigo, Ourense Campus, As Lagoas s/n, 32004 Ourense, Spain.

Abstract

In this study, probiotic biomass and nisin productions by Lactococcus lactis CECT 539 were followed in two realkalized fed-batch cultures in diluted whey (DW) supplemented with KH₂PO₄ up to a total phosphorus concentration of 0.459 g/L. Increased biomass (5.12 g/L, 2.18 × 10¹⁰ CFU/mL) and nisin (235.23 BU/mL) concentrations were obtained in the culture fed concentrated whey and concentrated mussel processing waste (CMPW) medium supplemented with glucose up to a concentration of 400 g/L (CMPW + G medium) compared with similar fed-batch fermentations in DW medium. In the second fed-batch fermentation, the feeding medium CMPW + G was supplemented with KH₂PO₄ up to a TP concentration of 3.21 g/L. With this approach, increased production of biomass (5.49 g/L, 2.33 × 10¹⁰ CFU/mL) and nisin (257.59 BU/mL) was obtained. Considering the substantial availability of these wastes at very low prices from local dairy and mussel processing plants in Galicia, their use as culture media could offer an attractive alternative for a low-cost production of probiotic biomass and nisin at a high scale.

Keywords: Fed-batch fermentation; Mussel processing waste; Nisin; Probiotic culture; Whey.