Differential release of eicosanoids by bradykinin, arachidonic acid and calcium ionophore A23187 in guinea-pig isolated perfused lung

Br J Pharmacol. 1985 Sep;86(1):55-62. doi: 10.1111/j.1476-5381.1985.tb09434.x.


The effects of infusions of bradykinin (0.2 microM), calcium ionophore A23187 (0.5 microM) and arachidonic acid (13 microM) on the release of eicosanoids from the guinea-pig isolated perfused lung were investigated using radioimmunoassay for thromboxane B2 (TXB2), 6-oxo-prostaglandin F1 alpha (6-oxo-PGF1 alpha), PGE2, leukotriene B4 (LTB4) and LTC4 and bioassay using the superfusion cascade. Bradykinin released more 6-oxo-PGF1 alpha than TXB2, whereas arachidonic acid and ionophore released more TXB2 than 6-oxo-PGF1 alpha. The time course of eicosanoid release varied with the stimulus: bradykinin and arachidonic acid produced an immediate release, whereas the ionophore showed a slower onset of release. Although the amounts of LTB4 and LTC4 released by the ionophore were very low according to radioimmunoassays, there was evidence from the bioassay of release of a leukotriene-like substance, thought to be LTD4. The leukotriene antagonist FPL 55712 lacks specificity in the guinea-pig trachea; at the concentration used (2 microM) it antagonized contractions of the tracheal strip to PGE2 as well as to LTC4. Our results show that in the guinea-pig perfused lung the metabolism of exogenous arachidonic acid is both qualitatively and quantitatively different from the metabolism of endogenous arachidonic acid; furthermore, the profile of eicosanoid production is stimulus-dependent.

Publication types

  • Comparative Study

MeSH terms

  • 6-Ketoprostaglandin F1 alpha / metabolism
  • Animals
  • Arachidonic Acid
  • Arachidonic Acids / pharmacology*
  • Bradykinin / pharmacology*
  • Calcimycin / pharmacology*
  • Chromones / pharmacology
  • Dinoprostone
  • Eicosanoic Acids / metabolism*
  • Guinea Pigs
  • In Vitro Techniques
  • Leukotriene B4 / metabolism
  • Lung / metabolism*
  • Male
  • Prostaglandins E / metabolism
  • SRS-A / metabolism
  • Thromboxane B2 / metabolism


  • Arachidonic Acids
  • Chromones
  • Eicosanoic Acids
  • Prostaglandins E
  • SRS-A
  • Leukotriene B4
  • Arachidonic Acid
  • Calcimycin
  • Thromboxane B2
  • 6-Ketoprostaglandin F1 alpha
  • FPL 55712
  • Dinoprostone
  • Bradykinin