Detecting RNA G-Quadruplexes (rG4s) in the Transcriptome

Cold Spring Harb Perspect Biol. 2018 Jul 2;10(7):a032284. doi: 10.1101/cshperspect.a032284.


RNA G-quadruplex (rG4) secondary structures are proposed to play key roles in fundamental biological processes that include the modulation of transcriptional, co-transcriptional, and posttranscriptional events. Recent methodological developments that include predictive algorithms and structure-based sequencing have enabled the detection and mapping of rG4 structures on a transcriptome-wide scale at high sensitivity and resolution. The data generated by these studies provide valuable insights into the potentially diverse roles of rG4s in biology and open up a number of mechanistic hypotheses. Herein we highlight these methodologies and discuss the associated findings in relation to rG4-related biological mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Algorithms
  • G-Quadruplexes*
  • Humans
  • RNA*
  • Sensitivity and Specificity
  • Transcriptome*


  • RNA