Chemogenetic control of gene expression and cell signaling with antiviral drugs

Nat Methods. 2018 Jul;15(7):519-522. doi: 10.1038/s41592-018-0042-y. Epub 2018 Jul 2.


We developed a method in which the NS3 cis-protease from hepatitis C virus can be used as a ligand-inducible connection to control the function and localization of engineered proteins in mammalian cells. To demonstrate the versatility of this approach, we designed drug-sensitive transcription factors and transmembrane signaling proteins, the activities of which can be tightly and reversibly controlled through the use of clinically tested antiviral protease inhibitors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antiviral Agents / pharmacology*
  • CHO Cells
  • Cricetulus
  • DNA / genetics
  • DNA / metabolism
  • Gene Expression Regulation / drug effects*
  • Signal Transduction
  • Viral Nonstructural Proteins / metabolism


  • Antiviral Agents
  • NS3 protein, hepatitis C virus
  • Viral Nonstructural Proteins
  • DNA