Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Dec;11(12):e201800174.
doi: 10.1002/jbio.201800174. Epub 2018 Jul 24.

Photobiomodulation Therapy Promotes in Vitro Wound Healing in Nicastrin KO HaCaT Cells

Affiliations

Photobiomodulation Therapy Promotes in Vitro Wound Healing in Nicastrin KO HaCaT Cells

Paola M Tricarico et al. J Biophotonics. .

Abstract

Mutations in NCSTN gene (encoding for nicastrin protein) are associated with hidradenitis suppurativa (HS), a chronic inflammatory disease involving hair follicles. HS is clinically handled with drugs but the most severe cases are treated with surgery. Photobiomodulation (PBM) therapy, already used in the treatment of skin diseases such as acne, herpes virus lesions, ultraviolet damage, vitiligo, hypertrophic scar, keloid, burn, psoriasis and diabetic chronic wounds, could be beneficial as an adjuvant supportive treatment to promote and foster the healing process after skin excision in HS. The effects of PBM therapy in promoting the wound closure are evaluated in a HaCaT cells NCSTN-/-, assessing cell metabolism, migration rate, proliferation and cell cycle progression. In our experimental model, PBM exerts a potent action on metabolism of mutated keratinocytes, incrementing adenosine triphosphate (ATP) production at 2 hours, while after 24 hours an increase of metabolism with a decrement of intracellular ATP levels were recorded. Moreover, PBM speeds up the wound closure, inducing cells' migration without affecting their proliferation.Based on our findings, we suggest the use of PBM in HS patients, who undergo major surgery with large skin excision.

Keywords: hidradenitis suppurativa; low-level light therapy; surgical wounds; wound healing.

Similar articles

See all similar articles

Publication types

MeSH terms

Substances

LinkOut - more resources

Feedback