In Vitro Study of Endotoxin Adsorption by a Polymyxin B-Immobilized Fiber Column

Blood Purif. 2018;46(4):269-273. doi: 10.1159/000489920. Epub 2018 Jul 3.


Background: Polymyxin B-immobilized fiber (PMX-F) columns are used as therapeutic interventions for septic shock. The clinical efficacy has been reported for 2-h applications, but their ability to adsorb endotoxin over longer treatments has not been fully elucidated. We hypothesized that PMX-F columns are capable of endotoxin removal for more than 2 h.

Method: We designed closed circuits incorporating either a PMX-F column with an 8.5-mL priming volume (PMX-01R) or a sham-control column, and used inactivated fetal bovine serum as the circulating perfusate. Endotoxin was continuously injected at a fixed rate for 24 h, and perfusate endotoxin concentrations were measured at fixed time points. PMX-01R endotoxin adsorption was calculated from the difference in the endotoxin concentrations.

Results: PMX-01R endotoxin adsorption increased continuously in a virtually linear manner.

Conclusions: The PMX-01R column showed sustained endotoxin adsorption for at least 24 h. This indicated that PMX-F columns would be capable of clinical endotoxin removal for 24 h.

Keywords: 24-h hemoperfusion; Adsorption saturation; Continuous endotoxin injection; Endotoxin adsorption; Polymyxin B-immobilized fiber.

MeSH terms

  • Endotoxins / blood*
  • Hemofiltration / instrumentation
  • Hemofiltration / methods*
  • Humans
  • Polymyxin B / chemistry*
  • Shock, Septic / blood
  • Shock, Septic / therapy


  • Endotoxins
  • Polymyxin B