Evaluation of a Hypertensive Rat Model Using Peripheral Blood Neutrophil Activity, Phagocytic Activity and Oxidized LDL Evaluation

Ran Zhang; Hiroyuki Inagawa; Kimiko Kazumura; Hiroshi Tsuchiya; Toshiyuki Miwa; Naokazu Morishita; Sachiko Uchibori; Jun Hanashiro; Tsutomu Masaki; Hideki Kobara; Gen-Ichiro Soma

doi:10.21873/anticanres.12726

Evaluation of a Hypertensive Rat Model Using Peripheral Blood Neutrophil Activity, Phagocytic Activity and Oxidized LDL Evaluation

Anticancer Res. 2018 Jul;38(7):4289-4294. doi: 10.21873/anticanres.12726.

Authors

Ran Zhang¹, Hiroyuki Inagawa^{2

3}, Kimiko Kazumura⁴, Hiroshi Tsuchiya⁴, Toshiyuki Miwa⁴, Naokazu Morishita⁵, Sachiko Uchibori², Jun Hanashiro², Tsutomu Masaki⁶, Hideki Kobara⁶, Gen-Ichiro Soma^{2

3}

Affiliations

¹ Control of Innate Immunity, Technology Research Association, Kagawa, Japan zhang@shizenmeneki.org.
² Control of Innate Immunity, Technology Research Association, Kagawa, Japan.
³ Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan.
⁴ Central Research Laboratory, Hamamatsu Photonics K.K., Hamamatsu, Japan.
⁵ Electron Tube Division, Hamamatsu Photonics K.K., Hamamatsu, Japan.
⁶ Department of Gastroenterology and Neurology, Faculty of Medicine, Kagawa University, Kagawa, Japan.

PMID: 29970563
DOI: 10.21873/anticanres.12726

Abstract

Background/aim: A system is being developed that can be used to easily evaluate the health condition of an individual with the help of trace amounts of a blood sample by focusing on xenobiotics. The system is called "Multimodal homeostasis evaluation system" (measurement of neutrophil activity, phagocytic activity of phagocytes and quantification of oxidized LDL (OxLDL)). To elucidate the possibility of using this system as an evaluation system for the health condition of an individual, clearly explaining the changes in various diseases is essential. In this study, evaluations were carried out using hypertensive model animals.

Materials and methods: Spontaneously hypertensive model rats SHR/NCrlCrlj and control rats WKY/NCrlCrlj were raised for 10 weeks from 6 to 16 weeks of age and their blood pressure was measured over time. Blood neutrophil activity (superoxide anion (O₂^•-) generation and myeloperoxidase (MPO) activity) and phagocytic activity of phagocytes was measured by our developed apparatus (a simple prototype device under development). OxLDL was measured by an ELISA kit, and biochemical markers were measured using the blood sample.

Results: Compared to WKY rats of the control group, systolic blood pressure, diastolic blood pressure, and mean blood pressure of SHR rats increased significantly with age. In SHR rats, there was a significant elevation in O₂^•- generation and MPO activity of neutrophils, alanine aminotransferase and triglycerides of blood, while phagocytic activity, OxLDL, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, total cholesterol and total-bilirubin decreased.

Conclusion: In the hypertensive model, biochemical markers were found to have a relationship with O₂^•- generation, MPO activity, phagocytic activity of phagocytes, and OxLDL. This system is expected to be useful for clinical monitoring of hypertension diseases.

Keywords: Peripheral blood; neutrophil activity; oxidized LDL; phagocytic activity.

MeSH terms

Animals
Biomarkers / blood*
Disease Models, Animal
Hypertension / blood*
Lipoproteins, LDL / analysis*
Male
Neutrophils*
Phagocytosis
Rats
Rats, Inbred SHR

Substances

Biomarkers
Lipoproteins, LDL
oxidized low density lipoprotein