Possible Application of Ascites-infiltrating Gamma-delta T Cells for Adoptive Immunotherapy

Anticancer Res. 2018 Jul;38(7):4327-4331. doi: 10.21873/anticanres.12732.

Abstract

Background/aim: Malignant ascites contain many tumour-infiltrating lymphocytes. γδ T cells with antitumour activity have attracted attention as effector cells in cancer immunotherapy. Vδ2+ T cells were cultured from peripheral blood mononuclear cells (PBMCs) and ascites-infiltrating lymphocytes (AILs) to compare the differences in response to 2-methyl-3-butenyl-1-pyrophosphate (2M3B1-PP) and zoledronate (Zol) as antigens in vitro.

Materials and methods: To expand Vδ2+ T cells from PBMCs and AILs from 29 patients with cancer, these cells were cultured and subjected to analysis.

Results: The proliferation rate of Vδ2+ T cells was higher in both PBMCs and AILs when cultured with Zol than with 2M3B1-PP. Although Vδ2+ T cells show a higher rate of expansion in AILs compared to PBMCs, the number of mixed tumour cells in ascites was decreased when cultured with Zol.

Conclusion: 2+ T cells in AILs are cytotoxic to tumour cells in ascites and may be considered in adoptive immunotherapy.

Keywords: ascites; ascites-infiltrating lymphocytes; ovarian cancer; tumour-infiltrating lymphocytes; γδ T cells.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Ascites / immunology
  • Ascitic Fluid / immunology
  • Female
  • Humans
  • Immunotherapy, Adoptive / methods*
  • Lymphocytes, Tumor-Infiltrating / immunology*
  • Middle Aged
  • Neoplasms / immunology
  • Receptors, Antigen, T-Cell, gamma-delta
  • T-Lymphocytes / immunology*

Substances

  • Receptors, Antigen, T-Cell, gamma-delta