DNA Organization and Superesolved Segregation

Methods Mol Biol. 2018:1805:271-289. doi: 10.1007/978-1-4939-8556-2_14.

Abstract

With single-molecule localization microscopy (SMLM) it is possible to reveal the internal composition, architecture, and dynamics of molecular machines and large cellular complexes. SMLM remains technically challenging, and frequently its implementation requires tailored experimental conditions that depend on the complexity of the subcellular structure of interest. Here, we describe two simple, robust, and high-throughput protocols to study molecular motors and machineries responsible for chromosome transport and organization in bacteria using 2D- and 3D-SMLM.

Keywords: Bacillus subtilis; Bacterial chromosome; Molecular motors; ParABS; Photoactivated localization microscopy (PALM); SpoIIIE; Sporulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus subtilis / metabolism*
  • DNA Replication
  • DNA, Bacterial / metabolism*
  • Imaging, Three-Dimensional
  • Microfluidics
  • Spores, Bacterial / metabolism

Substances

  • DNA, Bacterial