Molecular cloning of murine interferon gamma (MuIFN-gamma) cDNA and its expression in heterologous mammalian cells

J Interferon Res. Summer 1985;5(3):511-20. doi: 10.1089/jir.1985.5.511.

Abstract

A complete cDNA clone of murine interferon-gamma (MuIFN-gamma) was obtained by recombining two appropriate segments from partial cDNA clones originally identified by colony hybridization with rat IFN-gamma chromosomal gene fragments as probes. An expression vector was constructed in which the cDNA was placed under control of the SV40 early promoter. Transient expression of MuIFN-gamma was obtained by transformation of COS-1 cells. Subsequently, this interferon expression unit was linked to a vector containing a dihydrofolate reductase (DHFR) modular gene and used to transform DHFR(-)-CHO cells. Cell clones were selected that constitutively produce an interferon activity which by several criteria was found to be indistinguishable from natural, splenocyte-derived MuIFN-gamma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • Cell Transformation, Viral
  • Chlorocebus aethiops
  • Cloning, Molecular*
  • Cricetinae
  • Cricetulus
  • DNA / metabolism*
  • Female
  • Genetic Vectors
  • Interferon-gamma / genetics*
  • Kidney
  • Mice
  • Mice, Inbred Strains
  • Ovary
  • Promoter Regions, Genetic
  • Simian virus 40 / genetics
  • Transcription, Genetic*

Substances

  • Interferon-gamma
  • DNA