Incomplete neutralization of hepatitis A virus in vitro due to lipid-associated virions

J Gen Virol. 1985 Nov;66 ( Pt 11):2501-5. doi: 10.1099/0022-1317-66-11-2501.


Hepatitis A virus (HAV) released from infected BS-C-1 cells was incompletely neutralized when incubated with a variety of convalescent sera (non-neutralizable fraction of 17 to 32%). Chloroform extraction of virus resulted in a substantial reduction of the non-neutralizable fraction (to less than 1%), suggesting that non-neutralizable virions might be associated with lipids. Non-neutralizable HAV recovered from untreated cell culture supernatant fluids sedimented heterogeneously and less rapidly than normal virus in rate-zonal sucrose gradients and also banded at a lower density in CsCl (1.14 to 1.18 g/ml) than normal, neutralizable virus (1.32 g/ml). This bimodal distribution of HAV in CsCl gradients was confirmed by cDNA-RNA hybridization. Together, these observations suggest that a substantial proportion of HAV particles released from infected cells are lipid-associated and imply an important role for cell membranes in the assembly and release of HAV in vitro.

MeSH terms

  • Animals
  • Cell Line
  • Cell Membrane / physiology
  • Centrifugation, Density Gradient
  • Chlorocebus aethiops
  • Chloroform
  • Hepatitis A Antibodies
  • Hepatitis Antibodies / immunology*
  • Hepatovirus / immunology*
  • Hepatovirus / metabolism
  • Humans
  • Immune Sera / immunology
  • Lipid Metabolism
  • Lipids / physiology*
  • Neutralization Tests
  • Virion / metabolism


  • Hepatitis A Antibodies
  • Hepatitis Antibodies
  • Immune Sera
  • Lipids
  • Chloroform