Identification of the Missing Protein Hyaluronan Synthase 1 in Human Mesenchymal Stem Cells Derived from Adipose Tissue or Umbilical Cord

J Proteome Res. 2018 Dec 7;17(12):4325-4328. doi: 10.1021/acs.jproteome.8b00384. Epub 2018 Jul 6.


Currently, 14% of the human proteome is made up of proteins whose existence is not confirmed by mass spectrometry. We performed a proteomic profiling of human mesenchymal stem cells derived from adipose tissue or umbilical cord (PRIDE accession number: PXD009893) and identified peptides derived from 13 of such missing proteins. Remarkably, we found compelling evidence of the expression of hyaluronan synthase 1 (NX_Q92839-1) and confirmed its identification by the fragmentation of four heavy-labeled peptides that coeluted with their endogenous light counterparts. Our data also suggest that mesenchymal stem cells constitute a promising source for the detection of missing proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / cytology*
  • Humans
  • Hyaluronan Synthases / isolation & purification*
  • Mesenchymal Stem Cells / chemistry*
  • Peptides / analysis
  • Proteome / analysis
  • Umbilical Cord / cytology*


  • Peptides
  • Proteome
  • HAS1 protein, human
  • Hyaluronan Synthases