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. 2018 Jul 5;18(1):65.
doi: 10.1186/s12866-018-1207-7.

Influence of food matrix type on extracellular products of Vibrio parahaemolyticus

Rundong Wang  1 , Lijun Sun  2 , Yaling Wang  3 , Yijia Deng  1 , Zhijia Fang  1 , Ying Liu  1 , Qi Deng  1 , Dongfang Sun  1 , Ravi Gooneratne  4
Affiliations

Influence of food matrix type on extracellular products of Vibrio parahaemolyticus

Rundong Wang et al. BMC Microbiol. .

Abstract

Background: Two strains of Vibrio parahaemolyticus (ATCC 17802 and 33847) in shrimp, oyster, freshwater fish, pork, chicken and egg fried rice were evaluated for production of hemolysin and exoenzymes of potential importance to the pathogenicity of this bacterium.

Results: The two strains of V. parahaemolyticus produced hemolysin, gelatinase, caseinase, phospholipase, urease, DNase and amylase in selected food matrices. Significantly higher (p < 0.05) hemolytic activity was produced by V. parahaemolyticus in egg fried rice > shrimp > freshwater fish > chicken > oyster > pork. But the exoenzyme activities were not consistent with the hemolytic activity profile, being significantly higher (p < 0.05) in shrimp > freshwater fish > chicken > oyster > pork > egg fried rice. Filtrates of V. parahaemolyticus from shrimp, freshwater fish and chicken given intraperitoneally to adult mice induced marked liver and kidney damage and were highly lethal compared with the filtrates of V. parahaemolyticus from oyster > egg fried rice > pork.

Conclusion: From in vitro and in vivo tests, it appears that the food matrix type has a significant impact on the activity of extracellular products and the pathogenicity of V. parahaemolyticus. From a food safety aspect, it is important to determine which food matrices can stimulate V. parahaemolyticus to produce additional extracellular factors. This is the first report of non-seafood including freshwater fish and chicken contaminated with V. parahaemolyticus to have been shown to be toxic to mice in vivo.

Keywords: Extracellular products; Food matrices; Pathogenicity; Vibrio parahaemolyticus.

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Conflict of interest statement

Ethics approval and consent to participate

The animal work presented in this study was approved by the Animal Care and Welfare Committee of Guangdong Ocean University (SYXK 2014–0053).

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

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Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
Hemolytic activity of V. parahaemolyticus in different food matrices. Error bars represent standard deviations of mean values from triplicate experiments (Control groups excluded). Means with different lowercase letters were significantly different (p < 0.05) among different food matrices
Fig. 2
Fig. 2
Liver and kidney function indices detection. The mice were sacrificed after giving different food matrix filtrates intraperitoneally for 12 h. The test groups given filtrates of V. parahaemolyticus ATCC 17802 (or ATCC33847) samples, control groups given filtrates of foods that were not inoculated with V. parahaemolyticus. Means with asterisks (*) are significantly different (p < 0.05) from the respective controls.
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