Regulation of lipoxygenase-1 and Dectin-1 on interleukin-10 in mouse Aspergillus fumigatus keratitis

Int J Ophthalmol. 2018 Jun 18;11(6):905-909. doi: 10.18240/ijo.2018.06.02. eCollection 2018.


Aim: To investigate the regulation of lipoxygenase (LOX)-1 and Dectin-1 on interleukin-10 (IL-10) production in mice with Aspergillus fumigatus (A. fumigatus) keratitis.

Methods: The corneas of C57BL/6 mice were pretreated with LOX-1 inhibitor Poly(I) or Dectin-1 siRNA separately before the infection of A. fumigatus. Polymerase chain reaction (PCR) and Western blot were used to detect the expression of IL-10.

Results: The mRNA and protein expressions of IL-10 were significantly increased in mice with A. fumigatus keratitis. Compared with the group pretreated with sterile water before infection, Poly(I) pretreatment suppressed IL-10 expression significantly. Compared with the group pretreated with scrambled siRNA before infection, Dectin-1 siRNA pretreatment significantly reduced IL-10 expression in response to A. fumigatus infection.

Conclusion: LOX-1 and Dectin-1 regulate IL-10 production in mouse A. fumigatus keratitis.

Keywords: Aspergillus fumigatus; Dectin-1; interleukin-10; keratitis; lipoxygenase-1.