A Muscle-Specific Enhancer RNA Mediates Cohesin Recruitment and Regulates Transcription In trans

Mol Cell. 2018 Jul 5;71(1):129-141.e8. doi: 10.1016/j.molcel.2018.06.008.

Abstract

The enhancer regions of the myogenic master regulator MyoD give rise to at least two enhancer RNAs. Core enhancer eRNA (CEeRNA) regulates transcription of the adjacent MyoD gene, whereas DRReRNA affects expression of Myogenin in trans. We found that DRReRNA is recruited at the Myogenin locus, where it colocalizes with Myogenin nascent transcripts. DRReRNA associates with the cohesin complex, and this association correlates with its transactivating properties. Despite being expressed in undifferentiated cells, cohesin is not loaded on Myogenin until the cells start expressing DRReRNA, which is then required for cohesin chromatin recruitment and maintenance. Functionally, depletion of either cohesin or DRReRNA reduces chromatin accessibility, prevents Myogenin activation, and hinders muscle cell differentiation. Thus, DRReRNA ensures spatially appropriate cohesin loading in trans to regulate gene expression.

Keywords: Myogenin; cohesin; enhancer RNA; muscle gene transcription; non-coding RNA.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • Cell Cycle Proteins / biosynthesis*
  • Cell Cycle Proteins / genetics
  • Cell Differentiation
  • Chromatin / genetics
  • Chromatin / metabolism
  • Chromosomal Proteins, Non-Histone / biosynthesis*
  • Chromosomal Proteins, Non-Histone / genetics
  • Enhancer Elements, Genetic*
  • HEK293 Cells
  • Humans
  • Mice
  • Muscle, Skeletal / cytology
  • Muscle, Skeletal / metabolism*
  • MyoD Protein / biosynthesis
  • MyoD Protein / genetics
  • Myogenin / biosynthesis*
  • Myogenin / genetics
  • RNA, Untranslated / genetics
  • RNA, Untranslated / metabolism*
  • Transcription, Genetic*

Substances

  • Cell Cycle Proteins
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • MyoD Protein
  • MyoD1 myogenic differentiation protein
  • Myog protein, mouse
  • Myogenin
  • RNA, Untranslated
  • cohesins