Generation of a human induced pluripotent stem cell line, KSCBi003-A, from human adipose tissue-derived mesenchymal stem cells using a chromosomal integration-free system

Hye Young Choi; So-Jung Kim; Gue Youn Go; Ara Kwon; Young Sam Im; Hye-Yeong Ha; Jin Tae Hong; Ji-Won Jung; Soo Kyung Koo

doi:10.1016/j.scr.2018.06.003

Generation of a human induced pluripotent stem cell line, KSCBi003-A, from human adipose tissue-derived mesenchymal stem cells using a chromosomal integration-free system

Stem Cell Res. 2018 Aug:31:1-4. doi: 10.1016/j.scr.2018.06.003. Epub 2018 Jun 27.

Authors

Hye Young Choi¹, So-Jung Kim², Gue Youn Go², Ara Kwon², Young Sam Im², Hye-Yeong Ha², Jin Tae Hong³, Ji-Won Jung², Soo Kyung Koo⁴

Affiliations

¹ Division of Intractable Disease, National Center for Stem cell and Regenerative Medicine, Korea National Institute of Health, Chungcheongbuk-do, South Korea; College of Pharmacy, Chungbuk National University, Chungcheongbuk-do, South Korea.
² Division of Intractable Disease, National Center for Stem cell and Regenerative Medicine, Korea National Institute of Health, Chungcheongbuk-do, South Korea.
³ College of Pharmacy, Chungbuk National University, Chungcheongbuk-do, South Korea.
⁴ Division of Intractable Disease, National Center for Stem cell and Regenerative Medicine, Korea National Institute of Health, Chungcheongbuk-do, South Korea. Electronic address: skkoo@nih.go.kr.

PMID: 29979972
DOI: 10.1016/j.scr.2018.06.003

Abstract

We generated a human induced pluripotent stem cell (hiPSC) line, KSCBi003-A, from adipose tissue-derived mesenchymal stem cells (Ad-MSCs) using a Sendai virus-based gene delivery system. We confirmed that the KSCBi003-A has a normal karyotype and short tandem repeat (STR)-based identities that match the parent cells. We also confirmed that the cell line expresses pluripotent stem cell markers such as Nanog, OCT4, SSEA-4, TRA-1-60, and TRA-1-81. We also analyzed that the KSCBi003-A has an ability to differentiate three germ layers (ectoderm, mesoderm, endoderm). This cell line is registered and available at the National Stem Cell Bank, Korea National Institute of Health.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Humans
Induced Pluripotent Stem Cells / metabolism*
Mesenchymal Stem Cells / metabolism*