Topical application of Mentha piperita essential oil accelerates wound healing in infected mice model

Inflammopharmacology. 2019 Jun;27(3):531-537. doi: 10.1007/s10787-018-0510-0. Epub 2018 Jul 6.


This study was conducted to evaluate the effects of the prepared ointments from Mentha piperita essential oil (M. piperita) on wound healing in the infected mice models. Each circular full-thickness wound was inoculated with 25 × 107 units of Staphylococcus aureus and Pseudomonas aeruginosa bacteria strains. The tissue bacterial count, histological analyses and expression levels of IL-10, TNF-α, TGF-β1, IL-1β, CCL2, CXCL1, VEGF and FGF-2 were assessed to identify the different doses of M. piperita on wound healing. Total tissue bacterial count, edema and inflammation level were declined, but the migration of fibroblasts, collagen synthesis and re-epithelization were increased in treated animals with M. piperita. The expression levels of CCL2, CXCL1, IL-1β, TGF-β1 and IL-10 genes were up-regulated in the M. piperita-treated animals compared to the control group. While the expression of TNF-α, VEGF and FGF-2 was down-regulated in comparison to the control group. This study indicated that M. piperita can be used for treatment of the infected wound.

Keywords: Collagen synthesis; Infectious wound; Mentha piperita; Pro-inflammatory cytokine; Re-epithelization.

MeSH terms

  • Animals
  • Biomarkers / metabolism
  • Disease Models, Animal
  • Down-Regulation / drug effects
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Inflammation / drug therapy
  • Inflammation / immunology
  • Male
  • Mentha piperita / chemistry*
  • Mice
  • Mice, Inbred BALB C
  • Oils, Volatile / pharmacology*
  • Plant Extracts / pharmacology*
  • Up-Regulation / drug effects
  • Wound Healing / drug effects*


  • Biomarkers
  • Oils, Volatile
  • Plant Extracts