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Review
. 2018 Oct;115(10):2467-2478.
doi: 10.1002/bit.26790. Epub 2018 Aug 11.

Techniques for Chromosomal Integration and Expression Optimization in Escherichia Coli

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Review

Techniques for Chromosomal Integration and Expression Optimization in Escherichia Coli

Bingming Ou et al. Biotechnol Bioeng. .

Abstract

Due to the inherent expression stability and low metabolic burden to the host cell, the expression of heterologous proteins in the bacterial chromosome in a precise and efficient manner is highly desirable for metabolic engineering and live bacterial applications. However, obtaining suitable chromosome expression levels is particularly challenging. In this minireview, we briefly present the technologies available for the integration of heterologous genes into Escherichia coli chromosomes and strategies to optimize the expression levels of heterologous proteins.

Keywords: Escherichia coli (E. coli); chromosomal expression; chromosomal integration; expression cassette design; optimization; synthetic biology.

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