Cerebrospinal fluid mitochondrial DNA levels in patients with multiple sclerosis

Mult Scler. 2019 Oct;25(11):1535-1538. doi: 10.1177/1352458518786055. Epub 2018 Jul 9.


The role of cerebrospinal fluid (CSF) mitochondrial DNA (mtDNA) levels as biomarker in multiple sclerosis (MS) is unknown. We determined CSF mtDNA levels in a cohort of 237 individuals, including patients with MS and clinically isolated syndrome (CIS), inflammatory and non-inflammatory neurological controls, and cognitively healthy controls (HC). mtDNA concentration was measured by droplet digital polymerase chain reaction. CSF mtDNA levels were increased in all pathological conditions compared with HC, though no differences were observed between relapse-onset and progressive MS clinical forms, CIS patients and neurological controls. These findings do not support the determination of CSF mtDNA levels as a useful biomarker in MS clinical practice.

Keywords: Mitochondrial DNA; cerebrospinal fluid; droplet digital PCR; multiple sclerosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Case-Control Studies
  • DNA, Mitochondrial / cerebrospinal fluid*
  • Demyelinating Diseases / cerebrospinal fluid
  • Female
  • Humans
  • Male
  • Middle Aged
  • Multiple Sclerosis, Chronic Progressive / cerebrospinal fluid*
  • Multiple Sclerosis, Relapsing-Remitting / cerebrospinal fluid*
  • Polymerase Chain Reaction


  • DNA, Mitochondrial