Generation of Mutants of Nuclear-Encoded Plastid Proteins Using CRISPR/Cas9 in the Diatom Phaeodactylum tricornutum

Guillaume Allorent; Erika Guglielmino; Cécile Giustini; Florence Courtois

doi:10.1007/978-1-4939-8654-5_24

Generation of Mutants of Nuclear-Encoded Plastid Proteins Using CRISPR/Cas9 in the Diatom Phaeodactylum tricornutum

Methods Mol Biol. 2018:1829:367-378. doi: 10.1007/978-1-4939-8654-5_24.

Authors

Guillaume Allorent¹, Erika Guglielmino², Cécile Giustini², Florence Courtois²

Affiliations

¹ Laboratoire de Physiologie Cellulaire et Végétale, Centre National de la Recherche Scientifique, Institut National Recherche Agronomique, Commissariat à l'Energie Atomique et aux Energies Alternatives, CEA Grenoble, UMR5168, Université Grenoble Alpes, Grenoble, France. guillaume.allorent@cea.fr.
² Laboratoire de Physiologie Cellulaire et Végétale, Centre National de la Recherche Scientifique, Institut National Recherche Agronomique, Commissariat à l'Energie Atomique et aux Energies Alternatives, CEA Grenoble, UMR5168, Université Grenoble Alpes, Grenoble, France.

PMID: 29987734
DOI: 10.1007/978-1-4939-8654-5_24

Abstract

Genome modifications in microalgae are becoming a widespread and mandatory tool for research in both fundamental and applied biology. Among genome editing methods in these photosynthetic organisms, CRISPR/Cas9 offers a specific, powerful and efficient tool for genome engineering by inducing mutations in targeted regions of the genome. Here we described a protocol that allows the generation of knockout mutants by CRISPR/Cas9 in the diatom Phaeodactylum tricornutum using biolistic transformation.

Keywords: Biolistic transformation; CRISPR/Cas9; Genome editing; Phaeodactylum tricornutum.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biolistics / instrumentation
Biolistics / methods*
CRISPR-Cas Systems*
Cell Nucleus / genetics
Chloroplast Proteins / genetics*
Diatoms / genetics*
Gene Editing
Gene Knockout Techniques
Mutation*

Substances

Chloroplast Proteins