Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy

Nat Methods. 2018 Aug;15(8):601-604. doi: 10.1038/s41592-018-0052-9. Epub 2018 Jul 9.

Abstract

Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Humans
  • Intravital Microscopy / methods
  • Kinetics
  • Light
  • Luminescent Proteins / chemistry*
  • Luminescent Proteins / metabolism*
  • Microscopy, Fluorescence / methods
  • Nanotechnology
  • Photochemical Processes
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism
  • Spectrophotometry

Substances

  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • red fluorescent protein