Genome-wide C-SWAT library for high-throughput yeast genome tagging

Nat Methods. 2018 Aug;15(8):598-600. doi: 10.1038/s41592-018-0045-8. Epub 2018 Jul 9.


Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces cerevisiae open reading frames (ORFs). In 5,661 strains, we inserted an acceptor module after each ORF that can be efficiently replaced with tags or regulatory elements. We validated the library with targeted sequencing and tagged the proteome with bright fluorescent proteins to quantify the effect of heterologous transcription terminators on protein expression and to localize previously undetected proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • DNA, Fungal / genetics
  • Genome, Fungal*
  • Genomic Library*
  • High-Throughput Nucleotide Sequencing
  • Open Reading Frames
  • Proteome / genetics
  • Proteomics
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins / genetics
  • Sequence Analysis, DNA
  • Sequence Tagged Sites


  • DNA, Fungal
  • Proteome
  • Saccharomyces cerevisiae Proteins