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, 13 (7), e0200500
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Isolation and Purification of Antibacterial Compound From Streptomyces Levis Collected From Soil Sample of North India

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Isolation and Purification of Antibacterial Compound From Streptomyces Levis Collected From Soil Sample of North India

Vineeta Singh et al. PLoS One.

Abstract

During the screening programme for microbial cultures producing antimicrobial agents, an active microbial strain of Streptomyces was isolated from the agricultural soil of Narnaul, Haryana India. Physiological, biochemical characteristics and 16S ribosomal RNA sequence homology studies revealed that it was similar to Streptomyces levis (sequence similarity 100%). The microbial strain was submitted to Genomebio Technologies Pvt. Ltd., Pune, Maharashtra, India under Accession No. EU124569. The isolated strain was found to produce extracellular active compound showing strong antimicrobial activity against Klebsiella pneumoniae MTCC 109, Pseudomonas aeruginosa MTCC 741 and Staphylococcus aureus MTCC 96. The antibacterial compound was successfully isolated and purified. Structure elucidation of antibacterial metabolite with EI-MS/ HRMS showed molecular ion peak at m/z 686 [M+H]+. Whereas, elemental analysis of the said compound showed C = 61.31, H = 8.61, N = 2.04 and O = 28.02, and indicated a molecular formula of C35H59NO12. The presence of 'chromone' nucleus in the compound's chemical structure was confirmed by using 1HNMR studies. The present study reports the purification of potential antibacterial compound from Streptomyces levis isolated from the unexplored soil of north India and warrants for further characterization of this potential compound for optimum utilization for antimicrobial purposes.

Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Growth of S. levis on different growth medium, a: ISP-2, b: ISP-3, c: ISP-4, d: ISP-5, e: ISP-6, f: ISP-7, g: Mac Conkey Agar, h: Casein Agar, i: Citrate utilization.
Fig 2
Fig 2. Phylogenetic tree showing evolutionary relationship of Streptomyces levis with other members of Streptomyces along with their evolutionary distances.
Fig 3
Fig 3. HPLC profile showing the pure compound eluted at 14.43 min.
Fig 4
Fig 4. IR spectrum of the purified compound.
Fig 5
Fig 5. H-NMR of the purified compound.
Fig 6
Fig 6. 2,6-disubstituted chromone derivative.

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Grant support

The author (Dr. Vineeta Singh) sincerely acknowledges the Department of Science and Technology (DST), Ministry of Science and Technology, Government of India, New Delhi for the financial support provided in the form of Young Scientist Fellowship under Fast Track Scheme (NO.SR/FT/LS-190/2009). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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