Characterization, antioxidant and immunomodulatory potential on exopolysaccharide produced by wild type and mutant Weissella confusa strains

Bukola Adebayo-Tayo; Racheal Ishola; Titiloye Oyewunmi

doi:10.1016/j.btre.2018.e00271

Characterization, antioxidant and immunomodulatory potential on exopolysaccharide produced by wild type and mutant Weissella confusa strains

Biotechnol Rep (Amst). 2018 Jul 3:19:e00271. doi: 10.1016/j.btre.2018.e00271. eCollection 2018 Sep.

Authors

Bukola Adebayo-Tayo¹, Racheal Ishola¹, Titiloye Oyewunmi²

Affiliations

¹ Department of Microbiology, University of Ibadan, Ibadan, Oyo State, Nigeria.
² Immunology Unit, Department of Chemical Pathology, College of Medicine, University of Ibadan, Nigeria.

Abstract

Characterization, antioxidant and immunomodulatory potential of exopolysaccharide (EPS) produced by wild type and mutant Weissella confusa was investigated. The EPS production ranged from 5490.2 to 5580.7 mg/L. Wild type Weissella confusa (WWCEPS) had the highest EPS production. Eight (8) sugar moieties were present in the EPS. Galactose had the highest EPS composition (34.6 mg/100 g and 33.5 mg/100 g EPS) in Wild type Weissella confusa EPS (WWCEPS) and mutant Weissella confusa EPS (MWCEPS). Wild type Weissella confusa and mutant Weissella confusa EPS had antioxidant capacity. The scavenging assay for the antioxidant increased in a dose dependent (0.5-10 mg/mL) manner. Wild type Weissella confusa EPS had the highest 1,1-Diphemy 1-2-picryl-hydrazyl (DPPH) capacity, total antioxidant activity, hydrogen peroxide and reducing power activity (71%, 1.9%, 86.7% and 1.9%). The mice injected peritorially with mutant Weissella confusa EPS had the highest IgG and IgM (68-87 mg/dL and 64-70 mg/dL). IgA of the mice treated with Wild type Weissella confusa EPS increased from 67 to 73 mg/dL. Wild type and mutant Weissella confusa EPS had immunomodulatory activity on the treated mice.

Keywords: Antioxidant; Exopolysaccharide; Immunoglobulin; Immunomodulatory; Lactic acid bacteria.