The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates

J Biotechnol. 2018 Oct 10;283:22-27. doi: 10.1016/j.jbiotec.2018.07.009. Epub 2018 Jul 9.

Abstract

Pleurotus tuber-regium (Fr.) Singer, being a white-rot fungus, is widely used for food and medicine in the Asia-Pacific region. In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis. The present genomic data provides new insights for lignocellulose bioconversion of white-rot fungus for the genus Pleurotus.

Keywords: BUSCO (Benchmarking Universal Single-Copy Orthologs); Carbohydrate-active enzymes (CAZymes); Diploid genome; Illumina sequencing; Pleurotus tuber-regium; Sclerotia-forming; White-rot fungi.

MeSH terms

  • Base Composition
  • Biomass
  • Diploidy
  • Fungal Proteins / genetics*
  • Genome, Fungal
  • Lignin / metabolism*
  • Molecular Sequence Annotation
  • Mycelium / genetics
  • Mycelium / growth & development
  • Pleurotus / genetics*
  • Pleurotus / growth & development
  • Sequence Analysis, DNA / methods*
  • beta-Glucans / metabolism

Substances

  • Fungal Proteins
  • beta-Glucans
  • lignocellulose
  • Lignin