Generation of integration-free induced pluripotent stem cells from a patient with spina bifida

Stem Cell Res. 2018 Aug;31:27-30. doi: 10.1016/j.scr.2018.05.007. Epub 2018 May 17.

Abstract

A skin biopsy was obtained from a 14-year-old female patient with a history of Myelomeningocele. Dermal fibroblasts were isolated and reprogrammed with Sendai virus (SeV) vectors encoding OCT3/4, SOX2, KLF4, and c-MYC. The generated induced Pluripotent Stem Cell (iPSC) clones NTDi4_09A were free of genomically integrated reprogramming genes, had a stable normal karyotype and expressed pluripotency markers. The iPSCs formed teratomas in mice, which were differentiated towards derivatives of the three germ layers in vivo. This iPSC line offers a useful resource to study a genetic profile of a patient with spina bifida.

Publication types

  • Case Reports
  • Research Support, N.I.H., Extramural

MeSH terms

  • Female
  • Humans
  • Induced Pluripotent Stem Cells / metabolism*
  • Infant
  • Spinal Dysraphism / metabolism*
  • Spinal Dysraphism / pathology