A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain-Cell-Type Targeting and Functionality

Cell. 2018 Jul 12;174(2):465-480.e22. doi: 10.1016/j.cell.2018.06.035.


Modern genetic approaches are powerful in providing access to diverse cell types in the brain and facilitating the study of their function. Here, we report a large set of driver and reporter transgenic mouse lines, including 23 new driver lines targeting a variety of cortical and subcortical cell populations and 26 new reporter lines expressing an array of molecular tools. In particular, we describe the TIGRE2.0 transgenic platform and introduce Cre-dependent reporter lines that enable optical physiology, optogenetics, and sparse labeling of genetically defined cell populations. TIGRE2.0 reporters broke the barrier in transgene expression level of single-copy targeted-insertion transgenesis in a wide range of neuronal types, along with additional advantage of a simplified breeding strategy compared to our first-generation TIGRE lines. These novel transgenic lines greatly expand the repertoire of high-precision genetic tools available to effectively identify, monitor, and manipulate distinct cell types in the mouse brain.

Keywords: Cre; Flp; TIGRE; calcium sensor; cell type; channelrhodopsin; optogenetics; reporter; transgenic mice; voltage sensor.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Brain / cytology
  • Brain / metabolism*
  • Calcium / metabolism
  • Cell Line
  • Gene Knockout Techniques / methods*
  • Genes, Reporter*
  • In Situ Hybridization, Fluorescence
  • Light
  • Mice
  • Mice, Transgenic
  • Microscopy, Fluorescence
  • Neurons / metabolism
  • Optogenetics
  • RNA, Untranslated / genetics
  • Transgenes / genetics


  • Gt(ROSA)26Sor non-coding RNA, mouse
  • RNA, Untranslated
  • Calcium