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. 2018 May;480(1):173-176.
doi: 10.1134/S1607672918030146. Epub 2018 Jul 14.

Detection of Hispidin by a Luminescent System From Basidiomycete Armillaria Borealis

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Detection of Hispidin by a Luminescent System From Basidiomycete Armillaria Borealis

A P Puzyr et al. Dokl Biochem Biophys. .

Abstract

In in vitro experiments, the possibility of using a luminescent system extracted from the luminous fungus Armillaria borealis has been shown to detect and determine the concentration of hispidin. A linear dependence of the luminescent response on the content of hispidin in solutions in the concentration range of 5.4 × 10-5-1.4 × 10-2 µM was detected. The stability of the enzyme system and the high sensitivity of the bioluminescent reaction allows carrying out multiple measurements with the analyte detection limit of 1.3 × 10-11 g. The obtained results show the prospects of creating a rapid bioluminescent method for the analysis of medical substances or extracts from various biological objects for the presence of hispidin.

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