Biomarkers for Predicting Malignant Pleural Mesothelioma in a Mexican Population

Int J Med Sci. 2018 Jun 4;15(9):883-891. doi: 10.7150/ijms.23939. eCollection 2018.

Abstract

Background: Diagnosis of malignant pleural mesothelioma (MPM) remains a challenge, especially when resources in pathology are limited. The study aimed to evaluate cost-effective tumor markers to predict the probability of MPM in plasma samples in order to accelerate the diagnostic workup of the tissue of potential cases. Methods: We conducted a case-control study stratified by gender, which included 75 incident cases with MPM from three Mexican hospitals and 240 controls frequency-matched by age and year of blood drawing. Plasma samples were obtained to determine mesothelin, calretinin, and thrombomodulin using enzyme-linked immunosorbent assays (ELISAs). We estimated the performance of the markers based on the area under the curve (AUC) and predicted the probability of an MPM diagnosis of a potential case based on the marker concentrations. Results: Mesothelin and calretinin, but not thrombomodulin were significant predictors of a diagnosis of MPM with AUCs of 0.90 (95% CI: 0.85-0.95), 0.88 (95% CI: 0.82-0.94), and 0.51 (95% CI: 0.41-0.61) in males, respectively. For MPM diagnosis in men we estimated a true positive rate of 0.79 and a false positive rate of 0.11 for mesothelin. The corresponding figures for calretinin were 0.81 and 0.18, and for both markers combined 0.84 and 0.11, respectively. Conclusions: We developed prediction models based on plasma concentrations of mesothelin and calretinin to estimate the probability of an MPM diagnosis. Both markers showed a good performance and could be used to accelerate the diagnostic workup of tissue samples in Mexico.

Keywords: asbestos.; calretinin; diagnostic marker; mesothelin; mesothelioma.

MeSH terms

  • Aged
  • Biomarkers, Tumor / analysis*
  • Calbindin 2 / blood*
  • Case-Control Studies
  • Female
  • GPI-Linked Proteins / blood*
  • Humans
  • Lung Neoplasms
  • Male
  • Mesothelioma / blood
  • Mesothelioma / diagnosis*
  • Mexico
  • Middle Aged
  • Pleural Neoplasms / blood
  • Pleural Neoplasms / diagnosis*

Substances

  • Biomarkers, Tumor
  • Calbindin 2
  • GPI-Linked Proteins
  • mesothelin