The aim of this study was to measure and record the universal transmucosal abutment height, and then evaluate whether it influenced loosening of the abutment screw by analyzing the torque and detorque values after mechanical cycling. Thirty-six implants, model CM Unitite, with internal conical connections (3.5 × 10 mm) and respective universal prosthetic abutments (n = 36, 3.25 × 6 mm), were divided into three groups (n = 12 each) with respective transmucosal heights of 0.8, 3.5, and 5.5 mm. Insertion torque of 20 Ncm was used in accordance with the manufacturer's specifications. Afterward, the samples were submitted to fatigue tests consisting of 500,000 cycles at a frequency of 2Hz, a dynamic compressive load of 120N, and an angle of 30°. The detorque values were measured with a digital torque meter and tabulated to perform statistical analyses; a level of significance of 5% was adopted. The mean detorque values (SD) obtained were 22.83 (6.30), 22.5 (5.45), and 19.41 (4.69) Ncm for transmucosal abutments with heights of 0.8, 3.5, and 5.5 mm, respectively, and showed no statistically significant difference ( P = .262). The authors of this study concluded that the transmucosal height of prosthetic abutments submitted to mechanical fatigue did not influence the detorque values.