Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Sep 24;62(10):e00506-18.
doi: 10.1128/AAC.00506-18. Print 2018 Oct.

Pharmacodynamics of Daptomycin Against Enterococcus Faecium and Enterococcus Faecalis in the Murine Thigh Infection Model

Affiliations
Free PMC article

Pharmacodynamics of Daptomycin Against Enterococcus Faecium and Enterococcus Faecalis in the Murine Thigh Infection Model

James M Kidd et al. Antimicrob Agents Chemother. .
Free PMC article

Abstract

The Clinical and Laboratory Standards Institute (CLSI) daptomycin MIC susceptibility breakpoint for the treatment of enterococcal infections is ≤4 μg/ml. However, patients receiving daptomycin for the treatment of infections caused by enterococci with MICs of ≤4 μg/ml may experience treatment failures. We assessed the pharmacodynamics of daptomycin against enterococci in a neutropenic murine thigh infection model and determined the exposures necessary for bacteriostasis and a 1-log10-CFU reduction of Enterococcus faecalis and Enterococcus faecium We further characterized daptomycin efficacy at clinically achievable exposures. Six E. faecium and 6 E. faecalis isolates (daptomycin MICs, 0.5 to 32 μg/ml) were studied. Daptomycin was administered at various doses over 24 h to achieve area under the free drug concentration-time curve-to-MIC ratios (fAUC0-24/MIC) ranging from 1 to 148. Daptomycin regimens that simulate mean human exposures following doses of 6, 8, and 10 mg/kg of body weight/day were also studied. Efficacy was assessed by the differences in the number of log10 CFU per thigh at 24 h. The Hill equation was used to estimate the fAUC0-24/MIC required to achieve bacteriostasis and a 1-log10-CFU reduction. For E. faecium, a 1-log10-CFU reduction required an fAUC0-24/MIC of 12.9 (R2 = 0.71). For E. faecalis, a 1-log10-CFU reduction was not achieved, while the fAUC0-24/MIC required for stasis was 7.2 (R2 = 0.8). With a human-simulated regimen of 6 mg/kg/day, a 1-log10-CFU reduction was observed in 3/3 E. faecium isolates with MICs of <4 μg/ml and 0/3 E. faecium isolates with MICs of ≥4 μg/ml; however, a 1-log10-CFU reduction was not achieved for any of the 6 E. faecalis isolates. These results, alongside clinical data, prompt a reevaluation of the current breakpoint.

Keywords: Enterococcus; animal models; antibiotic resistance; breakpoints; susceptibility testing.

Figures

FIG 1
FIG 1
Dose versus fAUC0–24 in the murine thigh infection model after single doses of daptomycin.
FIG 2
FIG 2
Daptomycin human-simulated-regimen (HSR) free plasma concentration-time profile in the murine thigh infection model compared with a simulated human profile of 6 mg/kg/day at steady state. Data are means ± standard deviations.
FIG 3
FIG 3
Daptomycin human-simulated-regimen (HSR) free plasma concentration-time profile in the murine thigh infection model compared with a simulated human profile of 10 mg/kg/day at steady state. Data are means ± standard deviations.
FIG 4
FIG 4
Response of E. faecalis (EFC) to total daily doses of daptomycin (DAP) from 1 to 25 mg/kg at 24 h compared to the 0-h controls.
FIG 5
FIG 5
Curve of best fit to the fAUC0–24/MIC ratios and the change in the number of log10 CFU at 24 h for the composite of E. faecalis (EFC) isolates in neutropenic mice.
FIG 6
FIG 6
Response of E. faecalis (EFC) to three daptomycin human-simulated regimens at 24 h compared to the 0-h controls.
FIG 7
FIG 7
Response of E. faecium (EFM) to three daptomycin human-simulated regimens at 24 h compared to the 0-h controls.
FIG 8
FIG 8
Curve of best fit to the fAUC0–24/MIC ratios and change in the number of log10 CFU at 24 h for the composite of all E. faecium (EFM) isolates in neutropenic mice.

Similar articles

See all similar articles

Cited by 8 articles

See all "Cited by" articles

MeSH terms

Feedback