CD32 expression is associated to T-cell activation and is not a marker of the HIV-1 reservoir

Nat Commun. 2018 Jul 16;9(1):2739. doi: 10.1038/s41467-018-05157-w.

Abstract

CD32 has been shown to be preferentially expressed in latently HIV-1-infected cells in an in vitro model of quiescent CD4 T cells. Here we show that stimulation of CD4+ T cells with IL-2, IL-7, PHA, and anti-CD3/CD28 antibodies induces T-cell proliferation, co-expression of CD32 and the activation of the markers HLA-DR and CD69. HIV-1 infection increases CD32 expression. 79.2% of the CD32+/CD4+ T cells from HIV+ individuals under antiretroviral treatment were HLA-DR+. Resting CD4+ T cells infected in vitro generally results in higher integration of provirus. We observe no difference in provirus integration or replication-competent inducible latent HIV-1 in CD32+ or CD32- CD4+ T cells from HIV+ individuals. Our results demonstrate that CD32 expression is a marker of CD4+ T cell activation in HIV+ individuals and raises questions regarding the immune resting status of CD32+ cells harboring HIV-1 proviruses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Monoclonal / pharmacology
  • Antigens, CD / genetics
  • Antigens, CD / immunology
  • Antigens, Differentiation, T-Lymphocyte / genetics
  • Antigens, Differentiation, T-Lymphocyte / immunology
  • CD28 Antigens / antagonists & inhibitors
  • CD28 Antigens / genetics
  • CD28 Antigens / immunology
  • CD3 Complex / antagonists & inhibitors
  • CD3 Complex / genetics
  • CD3 Complex / immunology
  • CD4-Positive T-Lymphocytes / drug effects
  • CD4-Positive T-Lymphocytes / immunology*
  • CD4-Positive T-Lymphocytes / virology
  • Gene Expression
  • HIV Infections / genetics*
  • HIV Infections / immunology
  • HIV Infections / virology
  • HIV-1 / genetics
  • HIV-1 / immunology*
  • HLA-DR Antigens / genetics
  • HLA-DR Antigens / immunology
  • Host-Pathogen Interactions / immunology*
  • Humans
  • Interleukin-2 / pharmacology
  • Interleukin-7 / pharmacology
  • Lectins, C-Type / genetics
  • Lectins, C-Type / immunology
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / genetics*
  • Male
  • Middle Aged
  • Phytohemagglutinins / pharmacology
  • Primary Cell Culture
  • Proviruses / genetics
  • Proviruses / immunology
  • Receptors, IgG / genetics*
  • Receptors, IgG / immunology
  • Virus Integration

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, Differentiation, T-Lymphocyte
  • CD28 Antigens
  • CD3 Complex
  • CD69 antigen
  • HLA-DR Antigens
  • IL7 protein, human
  • Interleukin-2
  • Interleukin-7
  • Lectins, C-Type
  • Phytohemagglutinins
  • Receptors, IgG