High expression level of MMP9 is associated with poor prognosis in patients with clear cell renal carcinoma

PeerJ. 2018 Jul 4:6:e5050. doi: 10.7717/peerj.5050. eCollection 2018.


Matrix metallopeptidase 9 (MMP9) was found to be associated with tumor aggressiveness. In this study, we focused on the correlation between MMP9 expression and clear cell renal carcinoma (ccRCC). Through the Gene Expression Omnibus (GEO) database, the Cancer Genome Atlas (TCGA) database and immunohistochemical (IHC) staining, we observed that compared with adjacent normal renal tissues, in ccRCC tissues the mRNA and protein levels of MMP9 were enhanced, and the mRNA levels of GTP-binding protein smg p21B(RAP1B), B rapidly accelerated fibrosarcoma (RAF), methyl ethyl ketone2 (MEK2), extracellular regulated protein kinases1 (ERK1), ERK2, v-ets avian erythroblastosis virus E26 oncogene homolog1 (ETS1) and ETS2 also increased. The Kaplan-Meier survival analysis suggested that high MMP9 expression was an unfavorable prognostic biomarker for ccRCC patients. Our results indicated that the increased expression level of MMP9 in ccRCC may be due to the activation of the Mitogen-activated protein kinases (MAPK)/ERK signaling pathway, and MMP9 may be an attractive target for ccRCC therapy.

Keywords: ERK; MAPK; MMP9; ccRCC.

Grants and funding

This work was funded by the Youth scientific research project of Fujian Provincial Heath Department, China (2015-1-2), the International S&T Cooperation Program of China (ISTCP, 2016YFE0121900), the Natural Science Foundation of Fujian Province (2018J01723), the Educational and Scientific Research Project for Young Scholars in Fujian Province (JAT170136), the Scientific Research Innovation Team Construction Program of Fujian Normal University (IRTL1702), the Fujian Provincial Industrial Technology Development and Application project (2016Y01010210), Fujian Key Laboratories Funds (2015J1001), and FJNU Key Laboratories Construction Funds. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.