[The Study of Protective Effect of Paeoniflorin on Lung Injury in MRL/lpr Mice]

Sichuan Da Xue Xue Bao Yi Xue Ban. 2018 May;49(3):394-398.
[Article in Chinese]

Abstract

Objective: To investigate the protective effects of paeoniflorin on the lung injury in systemic lupus erythematosus with mouse model.

Methods: Ten wild type mice and 40 MRL/lpr mice were used in this study. MRL/lpr mice were randomly assigned to MRL/lpr group,MRL/lpr + dexamethasone (1.5 mg/kg) group,MRL/lpr + paeoniflorin (20 mg/kg) group,and MRL/lpr + paeoniflorin (40 mg/kg). The levels of malondialdehyde (MDA) , superoxide dismutase (SOD) ,catalase (CAT) ,glutathione peroxidase (GSH-Px) in serum were detected. The serum levesl of inflammatory cytokines were measured. Lung pathological changes were determined by HE staining. The protein level of phospho-phosphatidylinositol-3 kinase (P-PI3K),phospho-serine-threonine kinase B(P-Akt) ,phospho-nuclear factor kappa B (P-NF-κB),phospho-inhibitor of nuclear factor kappa Bα (P-IκBα) were detected by Western blot.

Results: Paeoniflorin decreased serum level of MDA and increased the levels of SOD,CAT,GSH-PX,and decreased the levels of inflammatory cytokines. Paeoniflorin improved lung pathological changes and inhibited the protein levels of P-PI3K,P-Akt,P-NF-κBp65,and P-IκBα in the lung tissue of MRL/lpr mice.

Conclusion: Paeoniflorin may be beneficial for the prevention of lung injury in systemic lupus erythematosus.

Keywords: Inflammation; Lung tissue; Paeoniflorin; Systemic lupus erythematosus.

MeSH terms

  • Animals
  • Glucosides / pharmacology*
  • Lung Injury / complications
  • Lung Injury / drug therapy*
  • Lupus Erythematosus, Systemic / complications
  • Lupus Erythematosus, Systemic / drug therapy*
  • Mice
  • Mice, Inbred MRL lpr
  • Monoterpenes / pharmacology*

Substances

  • Glucosides
  • Monoterpenes
  • peoniflorin