The levels of NF-κB p50 and NF-κB p65 play a role in thyroid carcinoma malignancy in vivo

J Int Med Res. 2018 Oct;46(10):4092-4099. doi: 10.1177/0300060518785846. Epub 2018 Jul 17.


Background To investigate the relationship between the levels of nuclear factor (NF)-κB p50 and NF-κB p65 and tumour characteristics in patients with thyroid carcinoma. Methods This prospective study enrolled consecutive patients with thyroid carcinoma. Tumour samples were collected and the levels of NF-κB p50 and NF-κB p65 protein and mRNA were measured using immunohistochemistry and quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). Results A total of 73 patients with thyroid carcinoma were included in the study (20 males; 53 females; mean ± SD age, 44.8 ± 12.7 years, range, 18-76 years). There were no significant differences in sex, age and pathological type between the NF-κB p50 positive group and the NF-κB p50 negative group, but tumour diameter and lymph node metastasis were significantly higher in the NF-κB p50 positive group compared with the NF-κB p50 negative group. Similar findings were observed for NF-κB p65. The levels of NF-κB p50 were positively correlated with NF-κB p65 in samples of thyroid carcinoma ( rs = 0.653). Conclusion The levels of NF-κB p50 and NF-κB p65 in samples of thyroid carcinoma were positively associated with tumour diameter and the presence of lymph node metastasis.

Keywords: Thyroid cancer; immunohistochemistry; nuclear factor-κB p50; nuclear factor-κB p65; quantitative real-time reverse transcription–polymerase chain reaction.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • China
  • Female
  • Humans
  • Immunohistochemistry
  • Lymphatic Metastasis
  • Male
  • Middle Aged
  • NF-kappa B p50 Subunit / blood*
  • Prospective Studies
  • RNA, Messenger / blood
  • Reverse Transcriptase Polymerase Chain Reaction
  • Thyroid Neoplasms / blood*
  • Thyroid Neoplasms / metabolism
  • Thyroid Neoplasms / pathology*
  • Transcription Factor RelA / blood*
  • Young Adult


  • NF-kappa B p50 Subunit
  • RNA, Messenger
  • Transcription Factor RelA