Upregulated structural and regulatory genes involved in anthocyanin biosynthesis for coloration of purple grains during the middle and late grain-filling stages

Abstract

Anthocyanin biosynthesis is controlled by structural and regulatory genes. Purple wheat grains accumulate anthocyanin during developmental processes. However, anthocyanin cannot accumulate at the beginning of grain formation. To understand the reason for this phenomenon, we performed observations and analyses of pigments, developmental stages, and the transcriptome of caryopsis in Triticum aestivum L. cv. Guizi 1 (GZ1). In the early grain-filling stage (10 dpa to 20 dpa), anthocyanin accumulated from nearly 0 mg·kg^-1 (10 dpa) to 15.39 mg·kg^-1 (20 dpa), and the expression levels of structural genes (except GzDFR) and main regulatory genes GzMYB-7D1 and GzMYC-2A1 were low. When the grains developed to the middle (20 dpa to 30 dpa) and late (30 dpa to 40 dpa) grain-filling stages, the anthocyanin content peaked at 197.31 mg·kg^-1, and the expression levels of structural and regulatory genes at 25 dpa and 35 dpa were higher than that at 10 dpa. In particular, the expression levels of GzANS, Gz3GT, GzMYB-7D1, and GzMYC-2A1 were upregulated 45.74˜28.54, 765.00˜384.00, 419.00˜574.00, and 5.34˜29.05 times, respectively. Grains were also colored from green to purple. Anthocyanin accumulates in the pericarp and testa and is stored in vacuoles of epidermal and transverse cells. The major compositions are cyanidin and peonidin. These results revealed that the upregulated structural and regulatory genes in the middle and late grain-filling stages may result in the anthocyanin biosynthesis and coloration of grains, which provides new insights into anthocyanin biosynthesis and regulation mechanisms.

Keywords: Anthocyanin; MYB; MYC; Purple wheat; Structural genes.