CalR: A Web-Based Analysis Tool for Indirect Calorimetry Experiments

Cell Metab. 2018 Oct 2;28(4):656-666.e1. doi: 10.1016/j.cmet.2018.06.019. Epub 2018 Jul 12.


We report a web-based tool for analysis of experiments using indirect calorimetry to measure physiological energy balance. CalR simplifies the process to import raw data files, generate plots, and determine the most appropriate statistical tests for interpretation. Analysis using the generalized linear model (which includes ANOVA and ANCOVA) allows for flexibility in interpreting diverse experimental designs, including those of obesity and thermogenesis. Users also may produce standardized output files for an experiment that can be shared and subsequently re-evaluated using CalR. This framework will provide the transparency necessary to enhance consistency, rigor, and reproducibility. The CalR analysis software will greatly increase the speed and efficiency with which metabolic experiments can be organized, analyzed per accepted norms, and reproduced and will likely become a standard tool for the field. CalR is accessible at

Keywords: ANCOVA; CLAMS; biostatistics; energy balance; energy expenditure; food intake; indirect calorimetry; metabolic phenotyping; metabolism; reproducibility.

Publication types

  • Research Support, American Recovery and Reinvestment Act
  • Research Support, N.I.H., Extramural

MeSH terms

  • Analysis of Variance
  • Animals
  • Calorimetry, Indirect / methods*
  • Cloud Computing*
  • Data Visualization
  • Energy Metabolism / physiology*
  • Humans
  • Linear Models
  • Mice
  • Obesity / metabolism
  • Pulmonary Gas Exchange / physiology
  • Reproducibility of Results
  • Thermogenesis
  • Web Browser*
  • Weight Loss / physiology
  • Workflow*