Synergistic effect of glucosamine and vitamin E against experimental rheumatoid arthritis in neonatal rats

Biomed Pharmacother. 2018 Sep;105:835-840. doi: 10.1016/j.biopha.2018.05.136. Epub 2018 Jun 18.


The effect of glucosamine and vitamin E against rheumatoid arthritis (RA) in a neonatal rat model was investigated. Lipid peroxidation, superoxide dismutase (SOD), catalase, reduced glutathione (GSH), glutathione peroxidase (Gpx), matrix metalloproteinase-3 (MMP-3), prostaglandin E2 (PGE2), ceruloplasmin, copper, zinc, nitric oxide (NO), uric acid, inducible nitric oxide synthase (iNOS), and nuclear factor-kappaB (NF-κB) levels were determined in control and rheumatoid arthritis neonatal rats. Glucosamine plus vitamin E supplementation reduced the MDA level by 61.9% and increased the SOD, catalase, GSH, Gpx, and zinc levels. MMP-3, PGE2, ceruloplasmin, copper, NO and uric acid levels were significantly reduced by supplementation of glucosamine plus vitamin E. NF-κB, and iNOS protein levels were decreased by 47.7% and 39.5%, respectively, by glucosamine plus vitamin E supplementation. Thus, supplementation with glucosamine plus vitamin E exerted a synergistic effect against RA.

Keywords: Glucosamine; Neonatal rats; Rheumatoid arthritis; Synergistic effect; Vitamin E.

MeSH terms

  • Animals
  • Animals, Newborn
  • Antioxidants / administration & dosage*
  • Arthritis, Rheumatoid / chemically induced
  • Arthritis, Rheumatoid / drug therapy*
  • Arthritis, Rheumatoid / metabolism*
  • Cells, Cultured
  • Drug Synergism
  • Drug Therapy, Combination
  • Freund's Adjuvant / toxicity
  • Glucosamine / administration & dosage*
  • Lipid Peroxidation / drug effects
  • Lipid Peroxidation / physiology
  • Male
  • Oxidative Stress / drug effects*
  • Oxidative Stress / physiology
  • Rats
  • Treatment Outcome
  • Vitamin E / administration & dosage*


  • Antioxidants
  • Vitamin E
  • Freund's Adjuvant
  • Glucosamine