Ciliostasis of airway epithelial cells facilitates influenza A virus infection

Vet Res. 2018 Jul 18;49(1):65. doi: 10.1186/s13567-018-0568-0.


Porcine precision-cut lung slices (PCLS) were used to analyze the effect of the ciliary activity on infection of airway epithelial cells by influenza viruses. Treatment of slices with 2% NaCl for 30 min resulted in reversible ciliostasis. When PCLS were infected by a swine influenza virus of the H3N2 subtype under ciliostatic conditions, the viral yield was about twofold or threefold higher at 24 or 48 h post-infection, respectively, as compared to slices with ciliary activity. Therefore, the cilia beating not only transports the mucus out of the airways, it also impedes virus infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cilia / pathology
  • Epithelial Cells / pathology
  • Epithelial Cells / virology
  • Influenza A Virus, H3N2 Subtype / physiology
  • Lung / physiopathology*
  • Lung / virology
  • Orthomyxoviridae Infections / physiopathology
  • Orthomyxoviridae Infections / veterinary*
  • Orthomyxoviridae Infections / virology
  • Swine
  • Swine Diseases / physiopathology
  • Swine Diseases / virology*