Screening of antifungal activity of 12 essential oils against eight pathogenic fungi of vegetables and mushroom

Lett Appl Microbiol. 2018 Oct;67(4):400-410. doi: 10.1111/lam.13053. Epub 2018 Aug 22.


The antifungal properties of 12 Eos, that is, Syzygium aromaticum, Pelargonium graveolens, Lavandula angustifolia, Cupresus sempervirens, Mentha piperita, Santolina chamaecyparissus, Citrus sinensis, Pogostemon patchouli, Thymus mastichina, Thymus vulgaris, Eucalyptus globulus and Rosmarinus officinalis, were screened. The influence of five doses of each EOs was tested against Botrytis cinerea, Sclerotinia sclerotiorum, Fusarium oxysporum, Phytophthora parasitica, Pythium aphanidermatum, Alternaria brassicae, Cladobotryum mycophilum and Trichoderma aggressivum f.sp. europaeum using disc-diffusion method. The mycelial growth inhibition and ED50 were calculated. The chemical analysis of the EOs was analysed using gas chromatography-mass spectroscopy. A total of 58 compounds were identified in the 12 EOs. All essential oils (EOs) analysed showed antifungal activity against the test pathogens in the range of 5·32-100%. The inhibitory effect of oils showed dose-dependent activity on the tested fungus. Based on the ED50 values, clove, rose geranium, peppermint and patchouli were the most effective. This study warrants further research into the practical use of EOs for the control of important myco- and phytopathogens in intensive horticulture.

Significance and impact of the study: Due to the serious damage caused by fungal pathogens of vegetables and mushrooms, it is necessary to search for integrated strategies of disease control. This study provides relevant information about the effects of 12 essential oils (EOs) against eight pathogens of agricultural interest, included mycopathogens with emphasis on the possible future application of the EOs as alternative antifungal agents.

Keywords: antifungal; biological control; essential oils; gas chromatography analysis; mushrooms; vegetables.

MeSH terms

  • Agaricales
  • Antifungal Agents / pharmacology*
  • Ascomycota / drug effects
  • Ascomycota / growth & development*
  • Botrytis / drug effects
  • Botrytis / growth & development*
  • Citrus sinensis / chemistry
  • Eucalyptus / chemistry
  • Fusarium / drug effects
  • Fusarium / growth & development*
  • Lavandula / chemistry
  • Microbial Sensitivity Tests
  • Oils, Volatile / pharmacology*
  • Pelargonium / chemistry
  • Plant Oils / pharmacology*
  • Pogostemon / chemistry
  • Syzygium / chemistry
  • Thymus Plant / chemistry
  • Vegetables / microbiology*


  • Antifungal Agents
  • Oils, Volatile
  • Plant Oils