Role of interleukin-17 in a murine community-associated methicillin-resistant Staphylococcus aureus pneumonia model

Microbes Infect. Jan-Feb 2019;21(1):33-39. doi: 10.1016/j.micinf.2018.06.006. Epub 2018 Jul 17.


Interleukin (IL)-17 is a key member of the Th17 cytokines and has been reported to be involved in the pathomechanisms underlying various diseases, including infectious diseases. Infections with community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) have garnered worldwide attention, and the representative USA300 strain is known to cause pneumonia in healthy people, which can be lethal. However, little is known about the role of IL-17 in CA-MRSA pneumonia. In this study, we investigated the role of IL-17 in a CA-MRSA pneumonia animal model. Mortality was higher and occurred at an earlier stage of infection in the IL-17A-knockout mice than in the wild-type (P < 0.01) and IL-17A/F-knockout mice (P < 0.05); however, no significant difference in the intrapulmonary bacterial counts was observed among the three groups of mice. Moreover, the IL-17A-knockout group showed significantly higher levels of IL-17F and granulocyte-colony stimulating factor (G-CSF) and a significantly higher neutrophil count in the bronchoalveolar lavage fluid than the other groups. These results confirmed that G-CSF expression significantly increased, and significant neutrophilic inflammation occurred under conditions of IL-17A deficiency in the murine CA-MRSA pneumonia model.

Keywords: IL-17; MRSA; Pneumonia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Models, Animal
  • Gene Expression
  • Granulocyte Colony-Stimulating Factor / genetics
  • Interleukin-17 / deficiency*
  • Interleukin-17 / genetics
  • Interleukin-17 / immunology*
  • Lung / immunology
  • Lung / microbiology
  • Male
  • Methicillin-Resistant Staphylococcus aureus / immunology*
  • Mice, Inbred BALB C
  • Mice, Knockout
  • Neutrophils / immunology
  • Pneumonia, Staphylococcal / immunology*
  • Pneumonia, Staphylococcal / microbiology
  • Pneumonia, Staphylococcal / mortality
  • Survival Rate


  • Il17a protein, mouse
  • Il17f protein, mouse
  • Interleukin-17
  • Granulocyte Colony-Stimulating Factor