Effect of Surface Modification on Viability of L929 Cells on Zirconia Nanocomposite Substrat

J Lasers Med Sci. 2018 Spring;9(2):87-91. doi: 10.15171/jlms.2018.18. Epub 2017 Mar 20.

Abstract

Introduction: Zirconia bioceramic can be considered for metallic replacement in dental implant applications. A proper method of surface modification may promote better osseointegration. Methods: In study evaluated viability of fibroblast cell following surface treatment. Therefore, viability L929 cells were characterized using MTT assay and scanning electron microscopy. Results: The viability assessment determined significant differences A-Y-TZP20 without surface treatment as compared to laser surface treatment (B), laser surface treatment + hydroxyapatiteyttrium stabilized tetragonal zirconia nanocomposite coat (C) and control. This study demonstrated that L929 cells approximately proliferated and spread on A-Y-TZP20 nanocomposite disk in laser surface treatment(B), Laser surface treatment + hydroxiapatite-yttrium stabilized tetragonal zirconia nanocomposite coat (C) groups similar to control group. Conclusion: Laser surface treatment showed positive effect on the viability of L929 cells.

Keywords: Alumina-yttrium stabilized tetragonal zirconia nanocomposite; Endosseous dental implant; Femtosecond laser; MTT assay.