Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
, 30 (5), 678-687

Hematology Reference Intervals for Adult Cows in France Using the Sysmex XT-2000iV Analyzer

Affiliations
Comparative Study

Hematology Reference Intervals for Adult Cows in France Using the Sysmex XT-2000iV Analyzer

Nicolas Herman et al. J Vet Diagn Invest.

Abstract

In order to develop bovine hematology reference intervals (RIs) in accordance with new international recommendations, we analyzed 156 blood specimens of healthy adult dairy and beef cows from 32 farms with a Sysmex XT-2000iV analyzer, and by manual scoring of platelet clumps and white blood cell (WBC) differential. We established RIs by the nonparametric method, and examined effects of age, production type (beef vs. dairy), and stage of lactation. RIs could not be determined for platelet count and indices because clumps were observed in 80% of specimens. Optical and impedance red blood cell (RBC) counts were similar, although statistically different. RIs for analyzer and manual WBC differentials were not different except for lymphocyte concentration, the subpopulations of which were counted manually. Hematocrit was higher in beef than dairy cattle, and hemoglobin was lower in early lactation. Increases in RBC count, mean corpuscular volume, mean corpuscular hemoglobin, and RBC distribution width were noted with increasing age, along with decreases in WBC count, neutrophils, and lymphocytes. Most RIs in our study, with the exception of neutrophils, were similar to those previously reported using a flow cytometry analyzer.

Keywords: CBC; Cattle; Sysmex hematology analyzer; hematology; leukocyte differential; reference intervals.

Conflict of interest statement

Declaration of conflicting interests: The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Figures

Figure 1.
Figure 1.
Bovine lymphocytes. a. Small lymphocytes (arrow) are 1–1.5 times the diameter of a red blood cell (RBC). b, e, g. Medium lymphocytes (white arrowhead) are 1.5–2.5 times the diameter of a RBC. c, d, g. Large lymphocytes (black arrowhead) are more than 2.5 times the diameter of a RBC. e, f. Granular lymphocytes (hatched arrow) can be small, medium, or large, with azurophilic granules in their cytoplasm. May-Grünwald/Giemsa. 1,000×.
Figure 2.
Figure 2.
Examples of the Sysmex XT-2000iV automated hematology analyzer histograms for bovine EDTA blood specimens. 1 = monocytes, 2 = lymphocytes, 3 = neutrophils, 4 = eosinophils, 5 = red blood cells, 6 = reticulocytes, and 7 = platelets. A. Typical “normal” graphs. B. Erroneous separation of the dot plots for neutrophils, lymphocytes, and monocytes in the WBC differential (DIFF) scattergram, and erroneous analyzer differential compared to the manual count. C. Abnormal gray dot plot highly suggestive of large platelet aggregates associated with erroneous platelet count in a cow. BASO = basophils; FSC = forward scatter; PLT = platelet count; PLT-O = PLT count by optical measurement; RBC = red blood cell count; RET = reticulocytes; SFL = side fluorescence; SSC = side scatter; WBC = white blood cell count.

Similar articles

See all similar articles

Publication types

LinkOut - more resources

Feedback