ALS-associated missense and nonsense TBK1 mutations can both cause loss of kinase function

Neurobiol Aging. 2018 Nov:71:266.e1-266.e10. doi: 10.1016/j.neurobiolaging.2018.06.015. Epub 2018 Jun 25.

Abstract

Mutations in TANK binding kinase 1 (TBK1) have been linked to amyotrophic lateral sclerosis. Some TBK1 variants are nonsense and are predicted to cause disease through haploinsufficiency; however, many other mutations are missense with unknown functional effects. We exome sequenced 699 familial amyotrophic lateral sclerosis patients and identified 16 TBK1 novel or extremely rare protein-changing variants. We characterized a subset of these: p.G217R, p.R357X, and p.C471Y. Here, we show that the p.R357X and p.G217R both abolish the ability of TBK1 to phosphorylate 2 of its kinase targets, IRF3 and optineurin, and to undergo phosphorylation. They both inhibit binding to optineurin and the p.G217R, within the TBK1 kinase domain, reduces homodimerization, essential for TBK1 activation and function. Finally, we show that the proportion of TBK1 that is active (phosphorylated) is reduced in 5 lymphoblastoid cell lines derived from patients harboring heterozygous missense or in-frame deletion TBK1 mutations. We conclude that missense mutations in functional domains of TBK1 impair the binding and phosphorylation of its normal targets, implicating a common loss of function mechanism, analogous to truncation mutations.

Keywords: ALS; FTD; Familial ALS; TBK1; WES.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyotrophic Lateral Sclerosis / genetics*
  • Cell Cycle Proteins
  • Codon, Nonsense
  • Exons
  • Female
  • Genetic Association Studies
  • Humans
  • Interferon Regulatory Factor-3 / genetics
  • Male
  • Membrane Transport Proteins
  • Mutation, Missense
  • Phosphorylation
  • Protein Binding
  • Protein Serine-Threonine Kinases / chemistry
  • Protein Serine-Threonine Kinases / genetics*
  • Protein Structure, Tertiary
  • Transcription Factor TFIIIA / genetics

Substances

  • Cell Cycle Proteins
  • Codon, Nonsense
  • IRF3 protein, human
  • Interferon Regulatory Factor-3
  • Membrane Transport Proteins
  • OPTN protein, human
  • Transcription Factor TFIIIA
  • Protein Serine-Threonine Kinases
  • TBK1 protein, human